pDL14
(Plasmid #106901)

• Purpose: pDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent manner
• Depositing Lab: Sergey Sineoky
• Publication: Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0167-7012(18)30368-3. doi: 10.1016/j.mimet.2018.06.001.

Backbone

• Vector backbone: pQE-30
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: pDL14 is conditionally replicating plasmid. The plasmid can not replicate in presense of IPTG or lactose.
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: gam
• Species: lambda phage
• Insert Size (bp): 417

Gene/Insert 2

• Gene/Insert name: bet
• Species: lambda phage
• Insert Size (bp): 786

Gene/Insert 3

• Gene/Insert name: mutS
• Species: Salmonella enterica LT2
• Insert Size (bp): 2568
• Mutation: Lysine 622 was replaced by Alanine

Gene/Insert 4

• Gene/Insert name: lacI
• Species: Escherichia coli
• Insert Size (bp): 1083

Gene/Insert 5

• Gene/Insert name: rhaR
• Species: Escherichia coli
• Insert Size (bp): 849

Gene/Insert 6

• Gene/Insert name: rhaS
• Species: Escherichia coli
• Insert Size (bp): 837

Resource Information

• Supplemental Documents:
  • pDL14

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry