Addgene - 1318 pcDNA3 flag FKHRL1 Plasmid Data

Plasmid 10708: 1318 pcDNA3 flag FKHRL1

Gene/insert name:
FKHRL1
Alt name:
FOXO3A
Insert size:
2000
Species:
H. sapiens (human)
Entrez Gene:
FOXO3 (AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A)
Fusion protein or tag:
flag
Terminal:
N terminal on backbone
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5446
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
William Sellers
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A novel mechanism of gene regulation and tumor suppression by the transcription factor FKHR. Ramaswamy et al (Cancer Cell. 2002 Jul . 2(1):81-91. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10708" in your Materials and Methods section.