Addgene - FHRE-Luc Plasmid Data

Plasmid 1789: FHRE-Luc

Gene/insert name:
FHRE
Alt name:
FKHRL1
Alt name:
FOXO3a
Alt name:
forkhead responsive element
Insert size:
96
Species:
H. sapiens (human)
Entrez Gene:
FOXO3 (AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A)
Mutation:
3 copies of FRE
Vector backbone:
pGL3 basic
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
5000
Cloning site 5':
NheI
Site destroyed during cloning:
No
Cloning site 3':
BglII
Site destroyed during cloning:
No
5' sequencing primer:
RVprimer3 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Michael Greenberg
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

See author's map and sequence.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor. Brunet et al (Cell 1999 Mar 19;96(6):857-68. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1789" in your Materials and Methods section.