Purposeexpresses V5-tagged miniTurbo in the mammalian cytosol
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||107170||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameminiTurbo (BirA mutant)
Mutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V160A, T192A, K194I, M209V, I305V
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Please visit https://www.biorxiv.org/content/early/2017/10/02/196980 for BioRxiv preprint
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:V5-miniTurbo-NES_pCDNA3 was a gift from Alice Ting (Addgene plasmid # 107170 ; http://n2t.net/addgene:107170 ; RRID:Addgene_107170)
For your References section:Efficient proximity labeling in living cells and organisms with TurboID. Branon TC, Bosch JA, Sanchez AD, Udeshi ND, Svinkina T, Carr SA, Feldman JL, Perrimon N, Ting AY. Nat Biotechnol. 2018 Aug 20. pii: nbt.4201. doi: 10.1038/nbt.4201. 10.1038/nbt.4201 PubMed 30125270