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Efficient proximity labeling in living cells and organisms with TurboID.
Branon TC, Bosch JA, Sanchez AD, Udeshi ND, Svinkina T, Carr SA, Feldman JL, Perrimon N, Ting AY
Nat Biotechnol. 2018 Aug 20. pii: nbt.4201. doi: 10.1038/nbt.4201.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
107167TurboID-V5_pRS415expresses V5-tagged TurboID in the yeast cytosol
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107168miniTurbo-V5_pRS415expresses V5-tagged miniTurbo in the yeast cytosol
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107169V5-TurboID-NES_pCDNA3expresses V5-tagged TurboID in the mammalian cytosol
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107170V5-miniTurbo-NES_pCDNA3expresses V5-tagged miniTurbo in the mammalian cytosol
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1071713xHA-TurboID-NLS_pCDNA3expresses 3xHA-tagged TurboID in the mammalian nucleus
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1071723xHA-miniTurbo-NLS_pCDNA3expresses 3xHA-tagged miniTurbo in the mammalian nucleus
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107173C1(1-29)-TurboID-V5_pCDNA3expresses V5-tagged TurboID on the mammalian ER membrane
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107174C1(1-29)-miniTurbo-V5_pCDNA3expresses V5-tagged miniTurbo on the mammalian ER membrane
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107175C1(1-29)-TurboID-V5_pLX304expresses V5-tagged TurboID on the mammalian ER membrane, lentiviral vector
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107176C1(1-29)-miniTurbo-V5_pLX304expresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vector
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107177TurboID-His6_pET21aexpresses His6-tagged TurboID in bacteria
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107178miniTurbo-His6_pET21aexpresses His6-tagged miniTurbo in bacteria
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1182203xHA-TurboID_pAS31expresses 3xHA-tagged TurboID in C. elegans intestine
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1182223xHA-miniTurbo_pAS32expresses 3xHA-tagged miniTurbo in C. elegans intestine
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1182233xHA-BioID_pAS28expresses 3xHA-tagged BioID in C. elegans intestine
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