Addgene - 884 pGEX2T PTEN G129E Plasmid Data

Plasmid 10747: 884 pGEX2T PTEN G129E

Gene/insert name:
PTEN
Insert size:
1200
Species:
H. sapiens (human)
Entrez Gene:
PTEN (10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, TEP1)
Fusion protein or tag:
GST
Terminal:
N terminal on backbone
Mutation:
G129E
Vector backbone:
pGEX-2T
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
4948
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
pGEX-5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
William Sellers
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Regulation of G1 progression by the PTEN tumor suppressor protein is linked to inhibition of the phosphatidylinositol 3-kinase/Akt pathway. Ramaswamy et al (Proc Natl Acad Sci U S A. 1999 Mar 2. 96(5):2110-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10747" in your Materials and Methods section.