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809 pcDNA3 GFP PTEN
(Plasmid #10759)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 10759 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PTEN
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1200
  • Entrez Gene
    PTEN (a.k.a. 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1)
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    809 pcDNA3 GFP PTEN was a gift from William Sellers (Addgene plasmid # 10759 ; http://n2t.net/addgene:10759 ; RRID:Addgene_10759)
  • For your References section:

    Phosphorylation of the PTEN tail acts as an inhibitory switch by preventing its recruitment into a protein complex. Vazquez F, Grossman SR, Takahashi Y, Rokas MV, Nakamura N, Sellers WR. J Biol Chem. 2001 Dec 28. 276(52):48627-30. 10.1074/jbc.C100556200 PubMed 11707428