AAV pCAG-mRuby3-WPRE
(Plasmid #107744)

• Purpose: Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.
• Depositing Lab: Rylan Larsen
• Publication: AAV red fluorescent protein plasmids (unpublished)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	107744	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: AAV pCAG-FLEX-tdTomato-WPRE (#51503)
  (Search Vector Database)
• Backbone manufacturer: Hongkui Zeng/Allen Institute For Brain Science
• Backbone size w/o insert (bp): 5396
• Total vector size (bp): 6270
• Modifications to backbone: A fragment containing mRuby3 was swapped into replace tdTomato and the LoxP sites in the original backbone.
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mRuby3
• Alt name: monomeric ruby3
• Alt name: mRuby3 red fluorescent protein
• Insert Size (bp): 874
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: TTCGGCTTCTGGCGTGTGAC
• 3′ sequencing primer: CCAGAGGTTGATTATCGATA

Resource Information

• Supplemental Documents:
  • Restriction Digest - SmaI
• A portion of this plasmid was derived from a plasmid made by: mRuby3 was synthesized de novo based on sequences published by Jun Chu's and Michael Lin's laboratories (Bajar et al, 2016, Nature Communications, PMID:26879144)
• Terms and Licenses:
  • UBMTA
• Industry Terms:
  • Not Available to Industry