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pNO286-3
(Plasmid #107746)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 107746 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pACYCDuet-1
  • Backbone size w/o insert (bp) 2550
  • Total vector size (bp) 5409
  • Modifications to backbone
    Medium copy p15A
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    CcaS
  • Alt name
    mini CcaS #10
  • Species
    Synthetic; Synechocystis sp. PCC 6803
  • Insert Size (bp)
    1389
  • Mutation
    Deleted AA 223 and 512
  • Promoter J23106

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Ho1
  • Species
    Synthetic; Synechocystis sp. PCC 6803
  • Insert Size (bp)
    723
  • GenBank ID
  • Promoter J23108

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    PcyA
  • Species
    Synthetic; Synechocystis sp. PCC 6803
  • Insert Size (bp)
    747
  • Promoter J23106

Cloning Information for Gene/Insert 3

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNO286-3 was a gift from Jeffrey Tabor (Addgene plasmid # 107746 ; http://n2t.net/addgene:107746 ; RRID:Addgene_107746)
  • For your References section:

    A miniaturized E. coli green light sensor with high dynamic range. Ong NTX, Tabor JJ. Chembiochem. 2018 Feb 8. doi: 10.1002/cbic.201800007. 10.1002/cbic.201800007 PubMed 29420866