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pACgp67B-Her2
(Plasmid #10794)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 10794 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pACgp67B
  • Backbone manufacturer
    Pharmingen
  • Backbone size w/o insert (bp) 9761
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ErbB2 ScFv
  • Alt name
    ML39 scFv
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    765
  • Entrez Gene
    ERBB2 (a.k.a. CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1)
  • Tag / Fusion Protein
    • 6His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site Bgl II (not destroyed)
  • 5′ sequencing primer polyhedrin forward
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACgp67B-Her2 was a gift from Judy Lieberman (Addgene plasmid # 10794 ; http://n2t.net/addgene:10794 ; RRID:Addgene_10794)
  • For your References section:

    Antibody mediated in vivo delivery of small interfering RNAs via cell-surface receptors. Song E, Zhu P, Lee SK, Chowdhury D, Kussman S, Dykxhoorn DM, Feng Y, Palliser D, Weiner DB, Shankar P, Marasco WA, Lieberman J. Nat Biotechnol. 2005 Jun . 23(6):709-17. 10.1038/nbt1101 PubMed 15908939