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HA.PKD.deltaPH
(Plasmid #10811)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 10811 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PKD
  • Species
    H. sapiens (human)
  • Mutation
    PH domain deleted (amino acids 417-545); F94I, V410G and D791G
  • Entrez Gene
    PRKD1 (a.k.a. CHDED, PKC-MU, PKCM, PKD, PRKCM)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The HA tag on this plasmid contains a point mutation that renders it unrecognizable by the 3F10 HA antibody. It will work fine with the 12CA5 HA antibody.

The PKD insert in this plasmid contains F94I, V410G and D791G mutations when compared to GenBank reference sequence NP_002733.1. Although the effect of these mutation are unknown, this is the exact plasmid used in the associated publication and it should function as described.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA.PKD.deltaPH was a gift from Alex Toker (Addgene plasmid # 10811)
  • For your References section:

    Tyrosine phosphorylation of protein kinase D in the pleckstrin homology domain leads to activation. Storz P, Doppler H, Johannes FJ, Toker A. J Biol Chem. 2003 May 16. 278(20):17969-76. 10.1074/jbc.M213224200 PubMed 12637538