PurposeUAS driven variant of iGluSnFR for zebrafish expression
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||108356||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2700
- Total vector size (bp) 5654
Vector typeZebrafish expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameUAS:[GlyRa1 secretion sequence]-[iGluSnFR]
Insert Size (bp)2954
- Promoter lac
- Cloning method Unknown
- 5′ sequencing primer M13 (Common Sequencing Primers)
Cloning was via SLiCE (Seamless Ligation Cloning Extract).
Note: The right tol1 region contains a single base pair mismatch compared to the depositor's expected sequence. The depositor has noted that this could reduce transgenesis efficiency, although they were able to successfully establish lines using the parent vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:UAS:iGluSnfr.zf1 was a gift from Harold Burgess (Addgene plasmid # 108356 ; http://n2t.net/addgene:108356 ; RRID:Addgene_108356)
For your References section:Presynaptic Inhibition Selectively Gates Auditory Transmission to the Brainstem Startle Circuit. Tabor KM, Smith TS, Brown M, Bergeron SA, Briggman KL, Burgess HA. Curr Biol. 2018 Aug 20;28(16):2527-2535.e8. doi: 10.1016/j.cub.2018.06.020. Epub 2018 Aug 2. 10.1016/j.cub.2018.06.020 PubMed 30078569