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pT2-CAG-fGFP
(Plasmid #108714)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 108714 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pT2HB
  • Backbone manufacturer
    Perry Hackett (Addgene plasmid 26557)
  • Modifications to backbone
    This plasmid was modified so that it included additional restriction sites and attR sites that would allow the insertion of sequences using Gateway cloning.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • Species
    Synthetic; Aequorea victoria (Jellyfish)
  • Promoter CAG
  • Tags / Fusion Proteins
    • farnesylation signal (C terminal on insert)
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The source plasmid with GFP was a gift from Safa Lucken-Ardjomande Häsler (member of the Harvey McMahon group at the MRC LMB). Dr. Häsler cloned the gene from the pEGFP-N3 plasmid, originally received from Clontech.
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT2-CAG-fGFP was a gift from Madeline Lancaster (Addgene plasmid # 108714 ; http://n2t.net/addgene:108714 ; RRID:Addgene_108714)
  • For your References section:

    Guided self-organization and cortical plate formation in human brain organoids. Lancaster MA, Corsini NS, Wolfinger S, Gustafson EH, Phillips AW, Burkard TR, Otani T, Livesey FJ, Knoblich JA. Nat Biotechnol. 2017 Jul;35(7):659-666. doi: 10.1038/nbt.3906. Epub 2017 May 31. 10.1038/nbt.3906 PubMed 28562594