|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22908||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeMammalian Expression, AAV
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namemouse alpha-calcium/calmodulin-dependent protein kinase II promoter
SpeciesM. musculus (mouse)
Insert Size (bp)3000
Entrez GeneCamk2a (a.k.a. CaMKII, R74975, mKIAA0968)
- Cloning method Restriction Enzyme
- 5′ sequencing primer Unknown
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Please note- this plasmid contains the mouse α-calcium/calmodulin-dependent protein kinase II promoter (mCAMK). There are several mismatches between depositor's sequence and Addgene's sequence. Please refer to Addgene's results for the correct sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-mCAMK-RFP was a gift from Edward Callaway (Addgene plasmid # 22908 ; http://n2t.net/addgene:22908 ; RRID:Addgene_22908)
For your References section:Preferential labeling of inhibitory and excitatory cortical neurons by endogenous tropism of adeno-associated virus and lentivirus vectors. Nathanson JL, Yanagawa Y, Obata K, Callaway EM. Neuroscience. 2009 Jun 30. 161(2):441-50. 10.1016/j.neuroscience.2009.03.032 PubMed 19318117