|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22910||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeMammalian Expression, AAV ; Adeno-associated virus
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameChicken Beta-actin promoter driving DsRedExpress
Insert Size (bp)3400
- Cloning method Restriction Enzyme
- 5′ sequencing primer Unknown
- 3′ sequencing primer SP6 (Common Sequencing Primers)
There are a few sequence mismatches with Chicken beta-actin promoter in BLAST, however this plasmid is functions as described in the associated publication.
Please note that this plasmid may have an alternative left ITR.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-CAG-RFP was a gift from Edward Callaway (Addgene plasmid # 22910 ; http://n2t.net/addgene:22910 ; RRID:Addgene_22910)
For your References section:Preferential labeling of inhibitory and excitatory cortical neurons by endogenous tropism of adeno-associated virus and lentivirus vectors. Nathanson JL, Yanagawa Y, Obata K, Callaway EM. Neuroscience. 2009 Jun 30. 161(2):441-50. 10.1016/j.neuroscience.2009.03.032 PubMed 19318117