Addgene - pMIG-Aalpha WT Plasmid Data

Plasmid 10884: pMIG-Aalpha WT

Gene/insert name:
PP2A Aalpha
Alt name:
PP2A
Species:
H. sapiens (human)
Entrez Gene:
PPP2R1A (PP2A-Aalpha, PP2AAALPHA, PR65A)
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Vector backbone:
pMIG
(Search Vector Database)
Backbone manufacturer:
Hahn lab, Addgene plasmid 9044
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
6500
Cloning site 5':
BglII
Site destroyed during cloning:
Yes
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
pLXSN 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
William Hahn
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Cancer-associated PP2A Aalpha subunits induce functional haploinsufficiency and tumorigenicity. Chen et al (Cancer Res. 2005 Sep 15. 65(18):8183-92. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10884" in your Materials and Methods section.