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pSEMS-CV SUy-Halo7Tag
(Plasmid #108933)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 108933 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSEMS(26m)
  • Backbone manufacturer
    Covalys, NEB
  • Backbone size w/o insert (bp) 5325
  • Total vector size (bp) 7026
  • Modifications to backbone
    original snap-Tag substituted by Halo7-Tag
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATP synthase F1 subunit gamma
  • Alt name
    ATP5F1C
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    891
  • GenBank ID
    NM_001001973.2
  • Entrez Gene
    ATP5F1C (a.k.a. ATP5C, ATP5C1, ATP5CL1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Halo7 tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer ATCGATATGTTCTCTCGCGCGGGTGTCGCTGGGCTGTCGGCC
  • 3′ sequencing primer GAAATTATCTCTGGTGCTGCAGCTCTGGCGGAATTCCG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSEMS-CV SUy-Halo7Tag was a gift from Karin Busch (Addgene plasmid # 108933 ; http://n2t.net/addgene:108933 ; RRID:Addgene_108933)
  • For your References section:

    Restricted diffusion of OXPHOS complexes in dynamic mitochondria delays their exchange between cristae and engenders a transitory mosaic distribution. Wilkens V, Kohl W, Busch K. J Cell Sci. 2013 Jan 1;126(Pt 1):103-16. doi: 10.1242/jcs.108852. Epub 2012 Oct 4. 10.1242/jcs.108852 PubMed 23038773