PurposeAnion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence in a viral vector
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||109048||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6686
- Total vector size (bp) 7823
Growth in Bacteria
Growth Strain(s)NEB Stable
Growth instructionsUse rec deficient competent cells such as SURE2
SpeciesM. musculus (mouse)
Insert Size (bp)1137
- Promoter CAG
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer Custom
- 3′ sequencing primer WPRE_R (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST was a gift from Hillel Adesnik (Addgene plasmid # 109048 ; http://n2t.net/addgene:109048 ; RRID:Addgene_109048)
For your References section:Precise multimodal optical control of neural ensemble activity. Mardinly AR, Oldenburg IA, Pegard NC, Sridharan S, Lyall EH, Chesnov K, Brohawn SG, Waller L, Adesnik H. Nat Neurosci. 2018 Apr 30. pii: 10.1038/s41593-018-0139-8. doi: 10.1038/s41593-018-0139-8. 10.1038/s41593-018-0139-8 PubMed 29713079
Map uploaded by the depositor.