pSV2 neuNT
(Plasmid #10918)

• Depositing Lab: Bob Weinberg
• Publication: Bargmann et al Cell. 1986 Jun 6. 45(5):649-57.

Backbone

• Vector backbone: pSV2 neo
• Backbone size w/o insert (bp): 4400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: neuNT
• Alt name: neu
• Alt name: p185
• Species: R. norvegicus (rat)
• Insert Size (bp): 5000
• Entrez Gene: Erbb2 (a.k.a. HER2/neu, neu)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: na

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See author's map. The neu cDNA was inserted between the HindIII and SmaI sites of pSV2-neo, removing the neo gene. Has normal neu cDNA as in pSV2 neuN with transforming sequence (1899-2387) of pSV2 neuT. Expressed in NIH cells at higher levels than pSV2 neuT.