|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10917||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepSV2 neo
- Backbone size w/o insert (bp) 4400
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Alt nameneu normal
SpeciesR. norvegicus (rat)
Insert Size (bp)5000
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
See author's map. The neu cDNA was inserted between the HindIII and SmaI sites of pSV2-neo, removing the neo gene. Expresses better than pSV2 neuT. Has about 100 nt of 5' untranslated region.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSV2 neuN was a gift from Bob Weinberg (Addgene plasmid # 10917 ; http://n2t.net/addgene:10917 ; RRID:Addgene_10917)
For your References section:Multiple independent activations of the neu oncogene by a point mutation altering the transmembrane domain of p185. Bargmann CI, Hung MC, Weinberg RA. Cell. 1986 Jun 6. 45(5):649-57. 10.1016/0092-8674(86)90779-8 PubMed 2871941
Map uploaded by the depositor.