|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||110568||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA
Backbone manufacturerKarl Deisseroth
- Backbone size w/o insert (bp) 5484
- Total vector size (bp) 6900
Vector typeMammalian Expression, AAV, Cre/Lox, Synthetic Biology
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert name3xFLAG/HA-tagged anti-GFP VHH
SpeciesLama pacos (camelid)
Insert Size (bp)456
- Promoter EF1a
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer Ef1a
- 3′ sequencing primer WPRE (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byVHH is from: Rothbauer, U., Zolghadr, K., Tillib, S., Nowak, D., Schermelleh, L., Gahl, A., Backmann, N., Conrath, K., Muyldermans, S., Cardoso, M.C., and Leonhardt, H. (2006). Targeting and tracing antigens in live cells with fluorescent nano- bodies. Nat. Methods 3, 887–889.
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-IV-3xFLAG/HA/NBL10 was a gift from Jeffrey Friedman & Alexander Nectow (Addgene plasmid # 110568 ; http://n2t.net/addgene:110568 ; RRID:Addgene_110568)