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pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNA
(Plasmid #110624)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 110624 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pX602 (Plasmid #61593)
  • Total vector size (bp) 6743
  • Modifications to backbone
    BsmBI sites near Amp cassette removed
  • Vector type
    Mammalian Expression, Mouse Targeting, AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    St1Cas9 LMD-9
  • Species
    H. sapiens (human), Synthetic
  • Promoter LP1B
  • Tags / Fusion Proteins
    • SV40 NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    sgRNA for St1Cas9
  • Species
    H. sapiens (human), Synthetic
  • Promoter hU6

Cloning Information for Gene/Insert 2

Resource Information

Depositor Comments

Mammalian codon-optimized Streptococcus thermophilus CRISPR1-Cas9 LMD-9 was subcloned from MSP1594 (Plasmid #65775). An SV40 NLS was added to the N-terminus. Please visit https://www.biorxiv.org/content/early/2018/05/14/321208 for BioRxiv preprint

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNA was a gift from Yannick Doyon (Addgene plasmid # 110624 ; http://n2t.net/addgene:110624 ; RRID:Addgene_110624)
  • For your References section:

    Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Agudelo D, Carter S, Velimirovic M, Duringer A, Rivest JF, Levesque S, Loehr J, Mouchiroud M, Cyr D, Waters PJ, Laplante M, Moineau S, Goulet A, Doyon Y. Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3. 10.1101/gr.255414.119 PubMed 31900288