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pALB-GFP
(Plasmid #55759)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 55759 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4151
  • Total vector size (bp) 6550
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    albumin enhancer/promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2335
  • Entrez Gene
    Alb (a.k.a. Alb-1, Alb1)
  • Promoter Mouse Albumin Enhancer/Promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer pBR322ori-F
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The mouse albumin enhancer/promoter fragment was cut out of p2335A-1, which was donated by Dr. Richard Palmiter. (Pinkert CA, Ornitz DM, Brinster RL, Palmiter RD. An albumin enhancer located 10 kb upstream functions along with its promoter to direct efficient,liver-specific expression in transgenic mice. Genes Dev. 1987 May;1(3):268-276.)
  • Terms and Licenses
  • Article Citing this Plasmid

Depositor Comments

The mouse albumin enhancer sequence extends from nt 59-2051, and the albumin promoter sequence consists of nt 2060-2394

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pALB-GFP was a gift from Snorri Thorgeirsson (Addgene plasmid # 55759 ; http://n2t.net/addgene:55759 ; RRID:Addgene_55759)
  • For your References section:

    Hepatic precursors derived from murine embryonic stem cells contribute to regeneration of injured liver. Heo J, Factor VM, Uren T, Takahama Y, Lee JS, Major M, Feinstone SM, Thorgeirsson SS. Hepatology. 2006 Dec;44(6):1478-86. 10.1002/hep.21441 PubMed 17133486