pALB-GFP
(Plasmid #55759)

• Purpose: Fluorescent marker for hepatocytic lineage
• Depositing Lab: Snorri Thorgeirsson
• Publication: Heo et al Hepatology. 2006 Dec;44(6):1478-86.

Backbone

• Vector backbone: pEGFP-1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4151
• Total vector size (bp): 6550
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: albumin enhancer/promoter
• Species: M. musculus (mouse)
• Insert Size (bp): 2335
• Entrez Gene: Alb (a.k.a. Alb-1, Alb1, BCL002)
• Promoter: Mouse Albumin Enhancer/Promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: pBR322ori-F
• 3′ sequencing primer: EGFP-N

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The mouse albumin enhancer/promoter fragment was cut out of p2335A-1, which was donated by Dr. Richard Palmiter. (Pinkert CA, Ornitz DM, Brinster RL, Palmiter RD. An albumin enhancer located 10 kb upstream functions along with its promoter to direct efficient,liver-specific expression in transgenic mice. Genes Dev. 1987 May;1(3):268-276.)
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The mouse albumin enhancer sequence extends from nt 59-2051, and the albumin promoter sequence consists of nt 2060-2394