|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31596||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerCalos Lab
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Gene/Insert namealpha-1 antitrypsin promoter with an apolipoprotein enhancer
SpeciesH. sapiens (human)
- Cloning method Restriction Enzyme
- 5′ sequencing primer hAAT-F
- 3′ sequencing primer Luc_N_Rev (Common Sequencing Primers)
As described in the methods:
pLiLucB is a liver-specific, luciferase-expressing attB donor plasmid that was constructed by digesting pNBL2 with XhoI to remove the CMV promoter and digesting pVFB with EcoRI to obtain the human alpha-1 antitrypsin promoter with an apolipoprotein enhancer. The ends of both fragments were made blunt by filling in with Klenow polymerase, and the pNBL2 backbone was treated with phosphatase. The construct was ligated using T4 ligase and checked by XcmI digestion and sequencing of the promoter region.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLiLucB was a gift from Michele Calos (Addgene plasmid # 31596 ; http://n2t.net/addgene:31596 ; RRID:Addgene_31596)
For your References section:Impact of hydrodynamic injection and phiC31 integrase on tumor latency in a mouse model of MYC-induced hepatocellular carcinoma. Woodard LE, Keravala A, Jung WE, Wapinski OL, Yang Q, Felsher DW, Calos MP. PLoS One. 2010 . 5(6):e11367. 10.1371/journal.pone.0011367 PubMed 20614008