pLiLucB
(Plasmid #31596)

• Depositing Lab: Michele Calos
• Publication: Woodard et al PLoS One. 2010 . 5(6):e11367.

Backbone

• Vector backbone: pNBL2
• Backbone manufacturer: Calos Lab
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: alpha-1 antitrypsin promoter with an apolipoprotein enhancer
• Species: H. sapiens (human)
• Entrez Gene: SERPINA1 (a.k.a. A1A, A1AT, AAT, PI, PI1, PRO2275, alpha1AT, nNIF)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: hAAT-F
• 3′ sequencing primer: Luc_N_Rev

Resource Information

• Supplemental Documents:
  • pLiLucB

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

As described in the methods:

pLiLucB is a liver-specific, luciferase-expressing attB donor plasmid that was constructed by digesting pNBL2 with XhoI to remove the CMV promoter and digesting pVFB with EcoRI to obtain the human alpha-1 antitrypsin promoter with an apolipoprotein enhancer. The ends of both fragments were made blunt by filling in with Klenow polymerase, and the pNBL2 backbone was treated with phosphatase. The construct was ligated using T4 ligase and checked by XcmI digestion and sequencing of the promoter region.