Addgene - pAL119-TK Plasmid Data

Plasmid 21911: pAL119-TK

Gene/insert name:
HSV Type 1 thymidine kinase
Alt name:
HSV1-TK
Alt name:
TK
Insert size:
1142
Species:
Herpes Simplex Type 1
GenBank ID:
FJ655139
Vector backbone:
pAL119
(Search Vector Database)
Vector type:
Adenoviral
Backbone size w/o insert (bp):
7337
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (2)
Map:
View map
Supplemental document:
pAL119tk Annotated Sequence (application/pdf)
Principal Investigator:
Maria Castro
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Chronic brain inflammation and persistent herpes simplex virus 1 thymidine kinase expression in survivors of syngeneic glioma treated by adenovirus-mediated gene therapy: implications for clinical trials. Dewey et al (Nat Med. 1999 Nov . 5(11):1256-63. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21911" in your Materials and Methods section.