|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21911||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7337
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameHSV Type 1 thymidine kinase
SpeciesHerpes Simplex Type 1
Insert Size (bp)1142
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Our diagnostic digest reveals a possible insertion within the backbone of this plasmid. It is unknown whether this discrepancy affects plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAL119-TK was a gift from Maria Castro (Addgene plasmid # 21911 ; http://n2t.net/addgene:21911 ; RRID:Addgene_21911)
For your References section:Chronic brain inflammation and persistent herpes simplex virus 1 thymidine kinase expression in survivors of syngeneic glioma treated by adenovirus-mediated gene therapy: implications for clinical trials. Dewey RA, Morrissey G, Cowsill CM, Stone D, Bolognani F, Dodd NJ, Southgate TD, Klatzmann D, Lassmann H, Castro MG, Loewenstein PR. Nat Med. 1999 Nov . 5(11):1256-63. 10.1038/15207 PubMed 10545991