Plasmid 9008: 1036 pcDNA3 Myr HA Akt1

• Gene/insert name: Akt1
• Alt name: Akt
• Insert size: 1500
• Species: H. sapiens (human)
• Entrez Gene: AKT1 (AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
  
• Fusion protein or tag: myr
• Terminal: N terminal on backbone
• Fusion protein or tag: HA
• Terminal: N terminal on backbone
• Vector backbone: pcDNA3
  (Search Vector Database)
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression
• Backbone size w/o insert: 5446
• Cloning site 5': BamHI
• Site destroyed during cloning: No
• Cloning site 3': EcoRI
• Site destroyed during cloning: No
• 5' sequencing primer: CMV-F
• 3' sequencing primer: BGH-rev
• Bacterial resistance(s) Ampicillin
• Growth strain(s) DH5alpha
• Growth temperature (℃): 37
• High or low copy: High Copy
• Selectable markers: Neomycin
• Sequence: View sequences (1)
• Map: View map
• Principal Investigator: William Sellers
• Terms and Licenses: MTA

Comments: 

The sequence of the HA tag in this plasmid is YAYDVPDYA instead of YPYDVPDYA. Not all HA antibodies will recognize this sequence. Cell Signaling Technology's HA antibody #2367 will work.

Article: Regulation of G1 progression by the PTEN tumor suppressor protein is linked to inhibition of the phosphatidylinositol 3-kinase/Akt pathway. Ramaswamy et al (Proc Natl Acad Sci U S A. 1999 Mar 2. 96(5):2110-5. PubMed)