Addgene - 896 pcDNA3 T7 Akt1 Plasmid Data

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Plasmid 9003: 896 pcDNA3 T7 Akt1

Gene/insert name:
Akt1
Alt name:
Akt
Insert size:
1500
Species:
H. sapiens (human)
Entrez Gene:
AKT1 (AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert:
5446
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
BGH-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
William Sellers
Terms and Licenses:
MTA

Comments:

Created by Francisca Vazquez. A cDNA for AKT-1 was PCR-amplified from a fetal brain library and ligated to the vector from pCDNA3-T7-VHL to give pCDNA3-T7-AKT.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Regulation of G1 progression by the PTEN tumor suppressor protein is linked to inhibition of the phosphatidylinositol 3-kinase/Akt pathway. Ramaswamy et al (Proc Natl Acad Sci U S A. 1999 Mar 2. 96(5):2110-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 9003" in your Materials and Methods section.