Addgene - 1477 pcDNA3 flag HA Akt1 Plasmid Data

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Plasmid 9021: 1477 pcDNA3 flag HA Akt1

Gene/insert name:
Akt1
Alt name:
Akt
Insert size:
1500
Species:
H. sapiens (human)
Entrez Gene:
AKT1 (AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
Fusion protein or tag:
flag
Terminal:
N terminal on backbone
Fusion protein or tag:
HA
Terminal:
N terminal on backbone
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert:
5446
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
BGH-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
William Sellers
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A library of siRNA duplexes targeting the phosphoinositide 3-kinase pathway: determinants of gene silencing for use in cell-based screens. Hsieh et al (Nucleic Acids Res. 2004 . 32(3):893-901. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 9021" in your Materials and Methods section.