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pLV CMV-TERRA
(Plasmid #111375)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 111375 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti4/V5-DEST
  • Backbone manufacturer
    Invitrogen
  • Modifications to backbone
    The inserted fragments of the pENTR plasmids were transferred by Gateway recombination cloning technique (Invitrogen) to lentiviral (pLenti6/BLOCK-iT-DEST and pLenti4/V5-DEST) expression vectors.
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TERRA polyA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    868
  • Promoter CMV

Cloning Information

Resource Information

Depositor Comments

The Addgene Partial NGS Result contains only a part (around 670 bps) of the 800 bps telomere sequence repeats, with further variant repeats not seen by Sanger sequencing by the Depositor

Please note that this plasmid may be prone to recombination due to the telomere hexanucleotide repeats, and therefore screening multiple colonies for the correct insert length is recommended before proceeding with downstream work

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV CMV-TERRA was a gift from Klaus Holzmann (Addgene plasmid # 111375 ; http://n2t.net/addgene:111375 ; RRID:Addgene_111375)
  • For your References section:

    Telomere Transcripts Target Telomerase in Human Cancer Cells. Kreilmeier T, Mejri D, Hauck M, Kleiter M, Holzmann K. Genes (Basel). 2016 Aug 16;7(8). pii: genes7080046. doi: 10.3390/genes7080046. 10.3390/genes7080046 PubMed 27537914