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pBB
(Plasmid #53210)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53210 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Agilent
  • Backbone size w/o insert (bp) 2948
  • Total vector size (bp) 3051

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TTAGGG(n) Track
  • Alt name
    Telomere Track
  • Alt name
    T2AG3 Track
  • Insert Size (bp)
    103
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (not destroyed)
  • 3′ cloning site BsmBI (not destroyed)
  • 5′ sequencing primer TAA TAC GAC TCA CTA TAG GG
  • 3′ sequencing primer AAT TAA CCC TCA CTA AAG GG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBB was a gift from Jack Griffith (Addgene plasmid # 53210 ; http://n2t.net/addgene:53210 ; RRID:Addgene_53210)
  • For your References section:

    TRF1 and TRF2 differentially modulate Rad51-mediated telomeric and nontelomeric displacement loop formation in vitro. Bower BD, Griffith JD. Biochemistry. 2014 Sep 2;53(34):5485-95. doi: 10.1021/bi5006249. Epub 2014 Aug 21. 10.1021/bi5006249 PubMed 25115914