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pSXneo 135(T2AG3)
(Plasmid #12402)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12402 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSP73
  • Backbone size w/o insert (bp) 2400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    135(T2AG3)
  • Alt name
    800bp TTAGGG repeats
  • Alt name
    138
  • Insert Size (bp)
    800

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (not destroyed)
  • 3′ cloning site Sal I (destroyed during cloning)
  • 5′ sequencing primer Sp6
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSXneo 135(T2AG3) was a gift from Titia de Lange (Addgene plasmid # 12402 ; http://n2t.net/addgene:12402 ; RRID:Addgene_12402)
  • For your References section:

    Stringent sequence requirements for the formation of human telomeres. Hanish JP, Yanowitz JL, de Lange T. Proc Natl Acad Sci U S A. 1994 Sep 13. 91(19):8861-5. 10.1073/pnas.91.19.8861 PubMed 8090736