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pZac2.1-GfaABC1D-mCherry-hPMCA2w/b
(Plasmid #111568)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 111568 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pZac2.1
  • Backbone size w/o insert (bp) 4454
  • Total vector size (bp) 9029
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Plasma membrane calcium pump 2, variant w/b
  • Alt name
    PMCA2w/b
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3732
  • GenBank ID
    NM_001036684.2
  • Entrez Gene
    ATP2B2 (a.k.a. PMCA2, PMCA2a, PMCA2i)
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Resource Information

Depositor Comments

This sequence for PMCA2w/b contains a G73D mutation compared to the reference but the depositing lab confirms that this mutation does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZac2.1-GfaABC1D-mCherry-hPMCA2w/b was a gift from Baljit Khakh (Addgene plasmid # 111568 ; http://n2t.net/addgene:111568 ; RRID:Addgene_111568)
  • For your References section:

    Reducing Astrocyte Calcium Signaling In Vivo Alters Striatal Microcircuits and Causes Repetitive Behavior. Yu X, Taylor AMW, Nagai J, Golshani P, Evans CJ, Coppola G, Khakh BS. Neuron. 2018 Aug 27. pii: S0896-6273(18)30688-3. doi: 10.1016/j.neuron.2018.08.015. 10.1016/j.neuron.2018.08.015 PubMed 30174118