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pAAV-hSynapsin1-axon-GCaMP6f
(Plasmid #112000)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 112000 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 4579
  • Total vector size (bp) 5989
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    axon-GCaMP6f
  • Alt name
    GAP43-GCaMP6f
  • Species
    Synthetic
  • Insert Size (bp)
    1410
  • GenBank ID
    MH282430
  • Promoter hSynapsin1
  • Tag / Fusion Protein
    • GAP43 palmitoylation domain (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer GAAGGCGCGCTGACGTCACTCGCCG
  • 3′ sequencing primer CAAATTTTGTAATCCAGAGGTTGATT
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSynapsin1-axon-GCaMP6f was a gift from Lin Tian (Addgene plasmid # 112000 ; http://n2t.net/addgene:112000 ; RRID:Addgene_112000)
  • For your References section:

    In vivo measurement of afferent activity with axon-specific calcium imaging. Broussard GJ, Liang Y, Fridman M, Unger EK, Meng G, Xiao X, Ji N, Petreanu L, Tian L. Nat Neurosci. 2018 Sep;21(9):1272-1280. doi: 10.1038/s41593-018-0211-4. Epub 2018 Aug 20. 10.1038/s41593-018-0211-4 PubMed 30127424