PurposeC:G-to-T:A base editing
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||112099||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)6378
- Promoter CMV
- Cloning method Unknown
- 5′ sequencing primer T7 Forward
- 3′ sequencing primer BGH-Reverse (Common Sequencing Primers)
Terms and Licenses
Addgene NGS results indicate a mixed population at position 5224. The majority of the population is the correct G nucleotide, although a minority shows an A at this position. Scientists may want to sequence this region of the plasmid prior to performing experiments.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV_BE4max_P2A_GFP was a gift from David Liu (Addgene plasmid # 112099 ; http://n2t.net/addgene:112099 ; RRID:Addgene_112099)
For your References section:Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Koblan LW, Doman JL, Wilson C, Levy JM, Tay T, Newby GA, Maianti JP, Raguram A, Liu DR. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. 10.1038/nbt.4172 PubMed 29813047