Addgene - pBABE-bleo IGF-1R Plasmid Data

Plasmid 11212: pBABE-bleo IGF-1R

Gene/insert name:
IGF-1R
Alt name:
IGFR
Alt name:
IGF-I receptor
Insert size:
4200
Species:
H. sapiens (human)
Entrez Gene:
IGF1R (CD221, IGFIR, IGFR, JTK13)
Vector backbone:
pBabe-bleo
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
4888
5' sequencing primer:
pBABE 5' List of Sequencing Primers
3' sequencing primer:
pBABE 3'
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Zeocin
Person or lab that originally
cloned the gene/insert:
Dr. Eva Surmacz
Sequence: View sequences (2)
Principal Investigator:
Ronald Kahn
Terms and Licenses:
MTA

Comments:

The insert was cloned from pcDNA IGF-1R (which contains a 4.2kb IGF-1R insert that lacks about 700bp of 3' sequence. This insert was cloned into the XhoI-XbaI sites of pcDNA). An EcoRI/XbaI fragment of pcDNA-IGF-1R was then inserted into SnaBI site of pBABE-bleo (EcoRI and XbaI sites were blunted).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Differential roles of the insulin and insulin-like growth factor-I (IGF-I) receptors in response to insulin and IGF-I. Entingh-Pearsall et al (J Biol Chem. 2004 Sep 3. 279(36):38016-24. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11212" in your Materials and Methods section.