Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pET21-HIS-Tol2
(Plasmid #112486)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 112486 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET21
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Tol2 Transposase
  • Species
    Synthetic
  • Insert Size (bp)
    2000
  • Entrez Gene
    tpase
  • Promoter T7
  • Tag / Fusion Protein
    • HIS/6x (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Term
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21-HIS-Tol2 was a gift from Stephen Ekker (Addgene plasmid # 112486 ; http://n2t.net/addgene:112486 ; RRID:Addgene_112486)
  • For your References section:

    Active recombinant Tol2 transposase for gene transfer and gene discovery applications. Ni J, Wangensteen KJ, Nelsen D, Balciunas D, Skuster KJ, Urban MD, Ekker SC. Mob DNA. 2016 Mar 31;7:6. doi: 10.1186/s13100-016-0062-z. eCollection 2016. 62 [pii] PubMed 27042235