pBABE-hygro CDK4 R24C
(Plasmid #11254)

• Depositing Lab: Bob Weinberg
• Publication: Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Backbone

• Vector backbone: pBABE-hygro
• Backbone size w/o insert (bp): 5558
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CDK4
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• Mutation: R24C
• Entrez Gene: CDK4 (a.k.a. CMM3, PSK-J3)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Resource Information

• Addgene Notes:
  • BamHI digest
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

R24C allele of the CDK4 gene encodes a kinase that is insensitive to inhibition by p16INK4A and p15INK4B, the major negative regulators of CDK4.