Addgene - pBABE-hygro CDK4 R24C Plasmid Data

Plasmid 11254: pBABE-hygro CDK4 R24C

Gene/insert name:
CDK4
Insert size:
1000
Species:
H. sapiens (human)
Entrez Gene:
CDK4 (CMM3, PSK-J3)
Mutation:
R24C
Vector backbone:
pBABE-hygro
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5558
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
pBABE 5' List of Sequencing Primers
3' sequencing primer:
pBABE 3'
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Hygromycin
Sequence: View sequences (2)
Map:
View map
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

R24C allele of the CDK4 gene encodes a kinase that is insensitive to inhibition by p16INK4A and p15INK4B, the major negative regulators of CDK4.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Enumeration of the simian virus 40 early region elements necessary for human cell transformation. Hahn et al (Mol Cell Biol 2002 Apr;22(7):2111-23. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11254" in your Materials and Methods section.