pBABE-hygro CDK4 R24C
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11254||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5558
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1000
Entrez GeneCDK4 (a.k.a. CMM3, PSK-J3)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer pBABE 5'
- 3′ sequencing primer pBABE 3' (Common Sequencing Primers)
R24C allele of the CDK4 gene encodes a kinase that is insensitive to inhibition by p16INK4A and p15INK4B, the major negative regulators of CDK4.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBABE-hygro CDK4 R24C was a gift from Bob Weinberg (Addgene plasmid # 11254 ; http://n2t.net/addgene:11254 ; RRID:Addgene_11254)
For your References section:Enumeration of the simian virus 40 early region elements necessary for human cell transformation. Hahn WC, Dessain SK, Brooks MW, King JE, Elenbaas B, Sabatini DM, DeCaprio JA, Weinberg RA. Mol Cell Biol 2002 Apr;22(7):2111-23. 10.1128/MCB.22.7.2111-2123.2002 PubMed 11884599