|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||112674||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backboneAdapted from lentiCRISPRv1
- Backbone size w/o insert (bp) 6296
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberLow Copy
Insert Size (bp)5331
- Promoter EF1s
- Cloning method Gibson Cloning
- 5′ sequencing primer TAAGTGCAGTAGTCGCCGTG
- 3′ sequencing primer TTAAGAATACCAGTCAATCTTTC (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti-ABE7.10-P2A-Puro was a gift from Lukas Dow (Addgene plasmid # 112674 ; http://n2t.net/addgene:112674 ; RRID:Addgene_112674)