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Ccas9LacZ
(Plasmid #113083)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 113083 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDD162 (Peft-3::Cas9 + Empty sgRNA, Addgene no.: #47549)
  • Modifications to backbone
    The original C. elegans Cas9 vector does not include any restriction enzyme cleavage site between the U6 promoter and gRNA sequence that would allow the spacer sequence to be efficiently modified. The redesigned vector (Ccas9lacZ) contains two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences which allow the spacer sequence to be easily modified with restriction digestion coupled linker ligation and homologous recombination-based cloning too. The new vector also contains a lacZ gene that allows blue-white selection after cloning.
  • Vector type
    Worm Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • Terms and Licenses

Depositor Comments

For plasmid usage, please see the associated publication: Sturm et al.
Highly efficient RNAi and Cas9-based auto-cloning systems for C. elegans research. Nucleic Acids Research, gky516, https://doi.org/10.1093/nar/gky516

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ccas9LacZ was a gift from Tibor Vellai (Addgene plasmid # 113083 ; http://n2t.net/addgene:113083 ; RRID:Addgene_113083)
  • For your References section:

    Highly efficient RNAi and Cas9-based auto-cloning systems for C. elegans research. Sturm A, Saskoi E, Tibor K, Weinhardt N, Vellai T. Nucleic Acids Res. 2018 Jun 19. pii: 5040046. doi: 10.1093/nar/gky516. 10.1093/nar/gky516 PubMed 29924347