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3X ERE TATA luc
(Plasmid #11354)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pGL2-TATA-Inr
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 5600
  • Vector type
    Bacterial Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    ERE
  • Species
    H. sapiens (human)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer lucNrev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Three copies of vitellogenin Estrogen Response Element (ERE). ERE sequence is: GGTCA CAG TGACC.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3X ERE TATA luc was a gift from Donald McDonnell (Addgene plasmid # 11354)
  • For your References section:

    The estrogen receptor beta-isoform (ERbeta) of the human estrogen receptor modulates ERalpha transcriptional activity and is a key regulator of the cellular response to estrogens and antiestrogens. Hall JM, McDonnell DP. Endocrinology. 1999 Dec . 140(12):5566-78. 10.1210/en.140.12.5566 PubMed 10579320