Addgene - 3X ERE TATA luc Plasmid Data

Plasmid 11354: 3X ERE TATA luc

Gene/insert name:
ERE
Species:
H. sapiens (human)
Vector backbone:
pGL2-TATA-Inr
(Search Vector Database)
Backbone manufacturer:
Stratagene
Vector type:
Bacterial Expression, Luciferase
Backbone size w/o insert (bp):
5600
Cloning site 5':
BglII
Site destroyed during cloning:
No
Cloning site 3':
BglII
Site destroyed during cloning:
No
5' sequencing primer:
lucNrev List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Donald McDonnell
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

Three copies of vitellogenin Estrogen Response Element (ERE). ERE sequence is: GGTCA CAG TGACC.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The estrogen receptor beta-isoform (ERbeta) of the human estrogen receptor modulates ERalpha transcriptional activity and is a key regulator of the cellular response to estrogens and antiestrogens. Hall et al (Endocrinology. 1999 Dec . 140(12):5566-78. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11354" in your Materials and Methods section.