3X ERE TATA luc
PurposeLuciferase reporter containing three copies of vitellogenin Estrogen Response Element
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11354||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5600
Vector typeBacterial Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer lucNrev (Common Sequencing Primers)
Three copies of vitellogenin Estrogen Response Element (ERE). ERE sequence is: GGTCA CAG TGACC.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:3X ERE TATA luc was a gift from Donald McDonnell (Addgene plasmid # 11354 ; http://n2t.net/addgene:11354 ; RRID:Addgene_11354)
For your References section:The estrogen receptor beta-isoform (ERbeta) of the human estrogen receptor modulates ERalpha transcriptional activity and is a key regulator of the cellular response to estrogens and antiestrogens. Hall JM, McDonnell DP. Endocrinology. 1999 Dec . 140(12):5566-78. 10.1210/endo.140.12.7179 PubMed 10579320