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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11350 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonena
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePRE
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SpeciesH. sapiens (human)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na
- 3′ sequencing primer lucNrev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Two copies of a consensus Progesterone Response Element (PRE) upstream of the thymidine kinase promoter.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
2X PRE TK luc was a gift from Donald McDonnell (Addgene plasmid # 11350 ; http://n2t.net/addgene:11350 ; RRID:Addgene_11350) -
For your References section:
The opposing transcriptional activities of the two isoforms of the human progesterone receptor are due to differential cofactor binding. Giangrande PH, Kimbrel EA, Edwards DP, McDonnell DP. Mol Cell Biol. 2000 May . 20(9):3102-15. 10.1128/MCB.20.9.3102-3115.2000 PubMed 10757795
