Addgene - PPRE X3-TK-luc Plasmid Data

Plasmid 1015: PPRE X3-TK-luc

Gene/insert name:
PPRE
Alt name:
PPRE-luc
Alt name:
DR1X3
Alt name:
PPAR response element
Insert size:
100
Species:
M. musculus (mouse)
Vector backbone:
ptkLUC (modified?)
(Search Vector Database)
Vector type:
Mammalian Expression, Luciferase
5' sequencing primer:
M13pUC-fwd List of Sequencing Primers
3' sequencing primer:
LucNrev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Bruce Spiegelman
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

3xDR-1 response elements (AGGACAAAGGTCA) upstream of a luciferase reporter.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: ADD1/SREBP1 activates PPARgamma through the production of endogenous ligand. Kim et al (Proc Natl Acad Sci U S A 1998 Apr 14;95(8):4333-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1015" in your Materials and Methods section.