Addgene - pSV Sport RXR alpha Plasmid Data

Plasmid 8882: pSV Sport RXR alpha

Gene/insert name:
RXR alpha
Alt name:
RXRa
Insert size:
1866
Species:
H. sapiens (human)
Entrez Gene:
RXRA (NR2B1)
Vector backbone:
pSV-Sport
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
3160
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
SP6 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
cDNA for hRXRa from Dr. Ron Evans (Mangelsdorf et al. 1990 Nature 345 p224)
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Bruce Spiegelman
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: mPPAR gamma 2: tissue-specific regulator of an adipocyte enhancer. Tontonoz et al (Genes Dev. 1994 May 15. 8(10):1224-34. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8882" in your Materials and Methods section.