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pcDNA flag PPAR gamma
(Plasmid #8895)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8895 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1 myc-His A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PPAR gamma
  • Alt name
    PPARg
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1547
  • GenBank ID
    NM_011146
  • Entrez Gene
    Pparg (a.k.a. Nr1c3, PPAR-gamma, PPAR-gamma2, PPARgamma, PPARgamma2)
  • Tag / Fusion Protein
    • flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA flag PPAR gamma was a gift from Bruce Spiegelman (Addgene plasmid # 8895 ; http://n2t.net/addgene:8895 ; RRID:Addgene_8895)
  • For your References section:

    Degradation of the peroxisome proliferator-activated receptor gamma is linked to ligand-dependent activation. Hauser S, Adelmant G, Sarraf P, Wright HM, Mueller E, Spiegelman BM. J Biol Chem. 2000 Jun 16. 275(24):18527-33. 10.1074/jbc.M001297200 PubMed 10748014