|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13458||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5010
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameRetinoic Acid Receptor Response Element
Alt nameRetinoic Acid Receptor Reporter
SpeciesM. musculus (mouse)
Insert Size (bp)80
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site NheI (destroyed during cloning)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer GLprimer2 (Common Sequencing Primers)
Please see author's map (link above) for a description of the insert.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL3-RARE-luciferase was a gift from T. Michael Underhill (Addgene plasmid # 13458 ; http://n2t.net/addgene:13458 ; RRID:Addgene_13458)
For your References section:BMP action in skeletogenesis involves attenuation of retinoid signaling. Hoffman LM, Garcha K, Karamboulas K, Cowan MF, Drysdale LM, Horton WA, Underhill TM. J Cell Biol. 2006 Jul 3. 174(1):101-13. 10.1083/jcb.200604150 PubMed 16818722