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pGL3-RARE-luciferase
(Plasmid #13458)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13458 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-promoter-luciferase
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5010
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Retinoic Acid Receptor Response Element
  • Alt name
    Retinoic Acid Receptor Reporter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    80

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site NheI (destroyed during cloning)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer GLprimer2
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please see author's map (link above) for a description of the insert.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-RARE-luciferase was a gift from T. Michael Underhill (Addgene plasmid # 13458)
  • For your References section:

    BMP action in skeletogenesis involves attenuation of retinoid signaling. Hoffman LM, Garcha K, Karamboulas K, Cowan MF, Drysdale LM, Horton WA, Underhill TM. J Cell Biol. 2006 Jul 3. 174(1):101-13. 10.1083/jcb.200604150 PubMed 16818722