pBS human IRS-1
(Plasmid #11359)

• Depositing Lab: Ronald Kahn
• Publication: Araki et al Diabetes. 1993 Jul . 42(7):1041-54.

Backbone

• Vector backbone: pBluescript II KS
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IRS-1
• Alt name: Insulin Receptor Substrate 1
• Alt name: IRS1
• Species: H. sapiens (human)
• Insert Size (bp): 5800
• GenBank ID: NM_005544
• Entrez Gene: IRS1 (a.k.a. HIRS-1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid contains full-length IRS-1 protein. Cloned from human muscle cDNA library. (Kahn lab plasmid name = phIRS1-1)