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pMH-Cas9-gate
(Plasmid #113742)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 113742 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEM-T easy
  • Backbone manufacturer
    Promega
  • Vector type
    CRISPR ; Gateway Destination Vector
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    May use DB3.1 cells instead of ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • Alt name
    SpCas9
  • Species
    S. pyogenes, Z. mays
  • Insert Size (bp)
    6197
  • Promoter Z. Mays ubiquitin promoter, 35S promoter

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

Also has hygromycinR driven by 35S promoter. We are not sure of the backbone -- it came from a plasmid that was made many many years ago. It aligns well with pGEM-T Easy (which a lot of our plasmids are based on) so if we had to guess, we would say the backbone is pGEM-T Easy.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMH-Cas9-gate was a gift from Magdalena Bezanilla (Addgene plasmid # 113742 ; http://n2t.net/addgene:113742 ; RRID:Addgene_113742)
  • For your References section:

    Efficient and modular CRISPR-Cas9 vector system for Physcomitrella patens. Mallett DR, Chang M, Cheng X, Bezanilla M. Plant Direct. 2019 Sep 12;3(9):e00168. doi: 10.1002/pld3.168. eCollection 2019 Sep. 10.1002/pld3.168 PubMed 31523744