pMK-Cas9-gate
(Plasmid #113743)

• Purpose: Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving aminoglycoside phosphotransferase for G418 selection in plants.
• Depositing Lab: Magdalena Bezanilla
• Publication: Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. doi: 10.1002/pld3.168. eCollection 2019 Sep.

Backbone

• Vector backbone: pGEM-T easy
• Backbone manufacturer: Promega
• Vector type: CRISPR ; Gateway Destination Vector
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: May use DB3.1 cells instead of ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cas9
• Alt name: SpCas9
• Species: S. pyogenes, Z. mays
• Insert Size (bp): 6203
• Promoter: Z. Mays ubiquitin promoter, 35S promoter

Cloning Information

• Cloning method: Gateway Cloning

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Also has aminoglycosyl phosphotransferase driven by 35S promoter (G418 resistance). We are not sure of the backbone -- it came from a plasmid that was made many many years ago. It aligns well with pGEM-T Easy (which a lot of our plasmids are based on) so if we had to guess, we would say the backbone is pGEM-T Easy.