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Plasmid 11508: pET32a-HD25Q
  • huntingtin, exon 1, 25 glutamines

  • huntingtin (Huntington disease)

  • 298

  • H. sapiens (human)

  • NM_002111

  • HTT (HD, IT15)

  • Trx

  • N terminal on backbone

  • His

  • C terminal on insert

  • His (out of frame)

  • C terminal on backbone

  • huntingtin exon 1 with 25 glutamines, vector modified-removed N-terminal His tag by digesting vector with MscI and NspV, filling in and blunt-end-ligating back together.

  • pET-32a
    (Search Vector Database)

  • Novagen

  • Bacterial Expression

  • 5900

  • NcoI

  • No

  • BamHI

  • No

  • T7 promoter List of Sequencing Primers

  • T7 terminator

  • Ampicillin

  • DH5alpha

  • 37

  • Unknown

  • A. Kazantsev

  • View sequences (1)
  • Pamela Bjorkman

  • UBMTA
    EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Inaugural Article: A linear lattice model for polyglutamine in CAG-expansion diseases. Bennett et al (Proc Natl Acad Sci U S A. 2002 Sep 3. 99(18):11634-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11508" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only