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pET32a-HD25Q
(Plasmid #11508)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pET-32a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    huntingtin, exon 1, 25 glutamines
  • Alt name
    huntingtin (Huntington disease)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    298
  • Mutation
    huntingtin exon 1 with 25 glutamines, vector modified-removed N-terminal His tag by digesting vector with MscI and NspV, filling in and blunt-end-ligating back together.
  • GenBank ID
    NM_002111
  • Entrez Gene
    HTT (a.k.a. HD, IT15)
  • Tags / Fusion Proteins
    • Trx (N terminal on backbone)
    • His (C terminal on insert)
    • His (out of frame) (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET32a-HD25Q was a gift from Pamela Bjorkman (Addgene plasmid # 11508)
  • For your References section:

    Inaugural Article: A linear lattice model for polyglutamine in CAG-expansion diseases. Bennett MJ, Huey-Tubman KE, Herr AB, West AP, Ross SA, Bjorkman PJ. Proc Natl Acad Sci U S A. 2002 Sep 3. 99(18):11634-9. 10.1073/pnas.182393899 PubMed 12193654