Plasmid 11516: pET32a-TRXtag
  • None - thioredoxin is vector encoded

  • trxA

  • TRX

  • E. coli thioredoxin

  • AAN83133

  • trxA ()

  • Trx

  • N terminal on backbone

  • His

  • C terminal on insert

  • His (out of frame)

  • C terminal on backbone

  • vector modified; removed N-terminal His tag by digesting vector with MscI and NspV, filling in and blunt-end-ligating back together.

  • pET-32a
    (Search Vector Database)

  • Novagen

  • Bacterial Expression

  • 5900

  • XbaI

  • No

  • BamHI

  • No

  • T7 promoter List of Sequencing Primers

  • T7 terminator

  • Ampicillin

  • DH5alpha

  • 37

  • Unknown

  • Novagen (vector derived)

  • View sequences (1)
  • Pamela Bjorkman

    EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Inaugural Article: A linear lattice model for polyglutamine in CAG-expansion diseases. Bennett et al (Proc Natl Acad Sci U S A. 2002 Sep 3. 99(18):11634-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11516" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only